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opn3 egfp reporter line  (Mutant Mouse Resource & Research Center)

 
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    Mutant Mouse Resource & Research Center opn3 egfp reporter line
    Immunohistochemistry analyzes of <t>Opn3,</t> Opn5, and Tubb3 expression in the nasal epithelium at E13.5 and E17.5 using Opn3 - <t>eGFP</t> and Opn5 -tdTomato mice. a–d) At E13.5, Opn3 is expressed in the Tubb3 + sensory epithelium ( N = 3), while no Opn5 expression is detected in the entire nasal epithelium ( N = 3). e–h) At E17.5, Opn3 is expressed in the Tubb3 + sensory epithelium ( N = 5), and Opn5 is expressed in a few scattered cells in the respiratory epithelium ( N = 5). White boxes indicate magnified images shown in (i–p). In (a, d, e and h), arrows indicate the vomeronasal organ, and arrowheads indicate migratory olfactory neurons. i–l) At e 17.5, Opn3 is co-localized with Tubb3, which marks postmitotic immature neurons, while no expression of Opn5 is detected in the sensory epithelium (SE). m–p) At E17.5, scattered Opn5 expression, but no Opn3 expression, is detected in the respiratory epithelium (RE; indicated with asterisks). Scale bars: a–d) 100 µm; e–h) 200 µm; i–p) 25 µm.
    Opn3 Egfp Reporter Line, supplied by Mutant Mouse Resource & Research Center, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/opn3+egfp+reporter+line/bac+d1+egfp+lines+reporter/pmc12628503-32-10-18
    Average 86 stars, based on 1 article reviews
    opn3 egfp reporter line - by Bioz Stars, 2026-09
    86/100 stars

    Images

    1) Product Images from "Opposing expression pattern of opsin 3 and opsin 5 in the developing and adult nasal epithelium"

    Article Title: Opposing expression pattern of opsin 3 and opsin 5 in the developing and adult nasal epithelium

    Journal: Chemical Senses

    doi: 10.1093/chemse/bjaf051

    Immunohistochemistry analyzes of Opn3, Opn5, and Tubb3 expression in the nasal epithelium at E13.5 and E17.5 using Opn3 - eGFP and Opn5 -tdTomato mice. a–d) At E13.5, Opn3 is expressed in the Tubb3 + sensory epithelium ( N = 3), while no Opn5 expression is detected in the entire nasal epithelium ( N = 3). e–h) At E17.5, Opn3 is expressed in the Tubb3 + sensory epithelium ( N = 5), and Opn5 is expressed in a few scattered cells in the respiratory epithelium ( N = 5). White boxes indicate magnified images shown in (i–p). In (a, d, e and h), arrows indicate the vomeronasal organ, and arrowheads indicate migratory olfactory neurons. i–l) At e 17.5, Opn3 is co-localized with Tubb3, which marks postmitotic immature neurons, while no expression of Opn5 is detected in the sensory epithelium (SE). m–p) At E17.5, scattered Opn5 expression, but no Opn3 expression, is detected in the respiratory epithelium (RE; indicated with asterisks). Scale bars: a–d) 100 µm; e–h) 200 µm; i–p) 25 µm.
    Figure Legend Snippet: Immunohistochemistry analyzes of Opn3, Opn5, and Tubb3 expression in the nasal epithelium at E13.5 and E17.5 using Opn3 - eGFP and Opn5 -tdTomato mice. a–d) At E13.5, Opn3 is expressed in the Tubb3 + sensory epithelium ( N = 3), while no Opn5 expression is detected in the entire nasal epithelium ( N = 3). e–h) At E17.5, Opn3 is expressed in the Tubb3 + sensory epithelium ( N = 5), and Opn5 is expressed in a few scattered cells in the respiratory epithelium ( N = 5). White boxes indicate magnified images shown in (i–p). In (a, d, e and h), arrows indicate the vomeronasal organ, and arrowheads indicate migratory olfactory neurons. i–l) At e 17.5, Opn3 is co-localized with Tubb3, which marks postmitotic immature neurons, while no expression of Opn5 is detected in the sensory epithelium (SE). m–p) At E17.5, scattered Opn5 expression, but no Opn3 expression, is detected in the respiratory epithelium (RE; indicated with asterisks). Scale bars: a–d) 100 µm; e–h) 200 µm; i–p) 25 µm.

    Techniques Used: Immunohistochemistry, Expressing

    Analyzes of Opn3 -eGFP/ Opn5 -tdTomato double reporter expression in comparison with Tubb3 and Foxj1 double immunohistochemistry at P14 ( N = 4). a–c) At P14, the Opn5 expression has expanded in the respiratory epithelium, with opposing Opn3 expression observed in the sensory epithelium. Most Opn5 + respiratory epithelial cells were observed in the distal part of the nasal cavity. A few intermingled Opn3 + and Opn5 + cells were detected where the sensory and respiratory domains meet (indicated by arrowheads). d) The sensory epithelium defined by Tubb3 expression and the respiratory domain by Foxj1 expression mimic the Opn3 and Opn5 expression domains, respectively. Scale bar: 200 µm.
    Figure Legend Snippet: Analyzes of Opn3 -eGFP/ Opn5 -tdTomato double reporter expression in comparison with Tubb3 and Foxj1 double immunohistochemistry at P14 ( N = 4). a–c) At P14, the Opn5 expression has expanded in the respiratory epithelium, with opposing Opn3 expression observed in the sensory epithelium. Most Opn5 + respiratory epithelial cells were observed in the distal part of the nasal cavity. A few intermingled Opn3 + and Opn5 + cells were detected where the sensory and respiratory domains meet (indicated by arrowheads). d) The sensory epithelium defined by Tubb3 expression and the respiratory domain by Foxj1 expression mimic the Opn3 and Opn5 expression domains, respectively. Scale bar: 200 µm.

    Techniques Used: Expressing, Comparison, Immunohistochemistry

    Immunohistochemistry analyzes of Opn3, Opn5, Foxj1, Ker8, Sox2, Tubb3, and OMP expression in the nasal epithelium at adulthood using Opn3 - eGFP ( N = 5) and Opn5 -tdTomato mice ( N = 4). a, b) In the adult nasal epithelium, an increased number of Opn5 + cells were detected in a scattered pattern within the Foxj1 + respiratory epithelium. White box in (a) indicates the magnified image shown in b. c–e) In the olfactory sensory epithelium, Opn3 and Ker8 are co-expressed in sustentacular cells. f) Opn3 is co-expressed with Sox2 in sustentacular cell nuclei (indicated by an arrow), but seemingly not in Sox2 + globose basal cells (indicated by arrowheads). g, h) Opn3 is not expressed in Tubb3 + postmitotic immature ORNs (g) or OMP + mature ORNs (h). Scale bars: a) 200 µm; b) 100 µm; c–h) 50 µm.
    Figure Legend Snippet: Immunohistochemistry analyzes of Opn3, Opn5, Foxj1, Ker8, Sox2, Tubb3, and OMP expression in the nasal epithelium at adulthood using Opn3 - eGFP ( N = 5) and Opn5 -tdTomato mice ( N = 4). a, b) In the adult nasal epithelium, an increased number of Opn5 + cells were detected in a scattered pattern within the Foxj1 + respiratory epithelium. White box in (a) indicates the magnified image shown in b. c–e) In the olfactory sensory epithelium, Opn3 and Ker8 are co-expressed in sustentacular cells. f) Opn3 is co-expressed with Sox2 in sustentacular cell nuclei (indicated by an arrow), but seemingly not in Sox2 + globose basal cells (indicated by arrowheads). g, h) Opn3 is not expressed in Tubb3 + postmitotic immature ORNs (g) or OMP + mature ORNs (h). Scale bars: a) 200 µm; b) 100 µm; c–h) 50 µm.

    Techniques Used: Immunohistochemistry, Expressing

    Buried food tests using Opn3 or Opn5 wild-type and deficient mice. Olfaction was evaluated by the buried food test, with a maximum of 10 min (i.e. 600 s) per trial to find a hidden cookie piece. a–c) The graphs show the time to find buried food for Opn3 +/+ ( N = 10) and Opn3 −/− mice ( N = 9). a) The bars represent the average time of three independent trials. b) The bars represent the three individual trials, T1–T3, analyzed as a function of trial order. c) The bars represent time to find buried food between T1 and T3 for Opn3 +/+ mice ( P = 0.0076), and Opn3 −/− mice ( P = 0.097). d–f) The graphs show the time to find buried food for Opn5 +/+ ( N = 8) and Opn5 −/− mice ( N = 11). (d) The bars represent the average time of three independent trials. e) The bars represent the three individual trials, T1–T3. f) The bars represent time to find buried food between T1 and T3 for Opn5 +/+ mice ( P = 0.72) and Opn5 −/− mice ( P = 0.21). Data on all graphs are presented as the mean ± standard deviation. Statistical significances were calculated using unpaired two-tailed Student's t -tests (a, c, d, f) or repeated measures two-way ANOVA followed by Sidak's multiple comparisons test (b, e).
    Figure Legend Snippet: Buried food tests using Opn3 or Opn5 wild-type and deficient mice. Olfaction was evaluated by the buried food test, with a maximum of 10 min (i.e. 600 s) per trial to find a hidden cookie piece. a–c) The graphs show the time to find buried food for Opn3 +/+ ( N = 10) and Opn3 −/− mice ( N = 9). a) The bars represent the average time of three independent trials. b) The bars represent the three individual trials, T1–T3, analyzed as a function of trial order. c) The bars represent time to find buried food between T1 and T3 for Opn3 +/+ mice ( P = 0.0076), and Opn3 −/− mice ( P = 0.097). d–f) The graphs show the time to find buried food for Opn5 +/+ ( N = 8) and Opn5 −/− mice ( N = 11). (d) The bars represent the average time of three independent trials. e) The bars represent the three individual trials, T1–T3. f) The bars represent time to find buried food between T1 and T3 for Opn5 +/+ mice ( P = 0.72) and Opn5 −/− mice ( P = 0.21). Data on all graphs are presented as the mean ± standard deviation. Statistical significances were calculated using unpaired two-tailed Student's t -tests (a, c, d, f) or repeated measures two-way ANOVA followed by Sidak's multiple comparisons test (b, e).

    Techniques Used: Standard Deviation, Two Tailed Test

    Immunohistochemistry analyzes of Tubb3, Foxj1, OMP, Sox2 and Ker8 in the nasal cavity of adult Opn3 +/+ , Opn3 −/− , Opn5 +/+ , and Opn5 −/− mice ( N = 3 for each mouse genotype). a–h) No apparent differences in the expression of Tubb3, Foxj1, OMP, Sox2, or Ker8 in the sensory epithelium of Opn3 −/− compared to Opn3 +/+ mice. e, f) Arrows indicate the Sox2 + sustentacular cells, and arrowheads indicate Sox2 + globose basal cells. i–l) No apparent difference in the expression of Tubb3, Foxj1, or Sox2 in the respiratory epithelium of Opn5 −/− compared to Opn5 +/+ mice. k, l) Arrows indicate the Sox2 + positive cells in the respiratory cell layer. Scale bars: a, b, i, j) 100 µm; c–h, k, l) 50 µm.
    Figure Legend Snippet: Immunohistochemistry analyzes of Tubb3, Foxj1, OMP, Sox2 and Ker8 in the nasal cavity of adult Opn3 +/+ , Opn3 −/− , Opn5 +/+ , and Opn5 −/− mice ( N = 3 for each mouse genotype). a–h) No apparent differences in the expression of Tubb3, Foxj1, OMP, Sox2, or Ker8 in the sensory epithelium of Opn3 −/− compared to Opn3 +/+ mice. e, f) Arrows indicate the Sox2 + sustentacular cells, and arrowheads indicate Sox2 + globose basal cells. i–l) No apparent difference in the expression of Tubb3, Foxj1, or Sox2 in the respiratory epithelium of Opn5 −/− compared to Opn5 +/+ mice. k, l) Arrows indicate the Sox2 + positive cells in the respiratory cell layer. Scale bars: a, b, i, j) 100 µm; c–h, k, l) 50 µm.

    Techniques Used: Immunohistochemistry, Expressing

    Related Articles

    Genetically Modified:

    Article Title: Opposing expression pattern of opsin 3 and opsin 5 in the developing and adult nasal epithelium
    Article Snippet: .. Genetically modified mouse strains used were as follows: (i) an Opn3 - eGFP reporter line ( Tg[Opn3-EGFP]JY3Gsat ; MMRRC/GENSAT stock number 030727-UCD, Mutant Mouse Resource & Research Centers, University of California, Davis, California); (ii) an Opn5 Cre/+ ; Ai14 reporter line ( ); (iii) an Opn3 lacZ/lacZ line that functionally acts like an Opn3 −/− line, mixed background strain C57Bl6/6N/FVB/N ( ); and (iv) an Opn5 −/− line, mixed background strain C57/129/CD1/FVB ( ). ..

    Mutagenesis:

    Article Title: Opposing expression pattern of opsin 3 and opsin 5 in the developing and adult nasal epithelium
    Article Snippet: .. Genetically modified mouse strains used were as follows: (i) an Opn3 - eGFP reporter line ( Tg[Opn3-EGFP]JY3Gsat ; MMRRC/GENSAT stock number 030727-UCD, Mutant Mouse Resource & Research Centers, University of California, Davis, California); (ii) an Opn5 Cre/+ ; Ai14 reporter line ( ); (iii) an Opn3 lacZ/lacZ line that functionally acts like an Opn3 −/− line, mixed background strain C57Bl6/6N/FVB/N ( ); and (iv) an Opn5 −/− line, mixed background strain C57/129/CD1/FVB ( ). ..



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    opn3 egfp reporter line  (Mutant Mouse Resource & Research Center)
    86
    Mutant Mouse Resource & Research Center opn3 egfp reporter line
    Immunohistochemistry analyzes of <t>Opn3,</t> Opn5, and Tubb3 expression in the nasal epithelium at E13.5 and E17.5 using Opn3 - <t>eGFP</t> and Opn5 -tdTomato mice. a–d) At E13.5, Opn3 is expressed in the Tubb3 + sensory epithelium ( N = 3), while no Opn5 expression is detected in the entire nasal epithelium ( N = 3). e–h) At E17.5, Opn3 is expressed in the Tubb3 + sensory epithelium ( N = 5), and Opn5 is expressed in a few scattered cells in the respiratory epithelium ( N = 5). White boxes indicate magnified images shown in (i–p). In (a, d, e and h), arrows indicate the vomeronasal organ, and arrowheads indicate migratory olfactory neurons. i–l) At e 17.5, Opn3 is co-localized with Tubb3, which marks postmitotic immature neurons, while no expression of Opn5 is detected in the sensory epithelium (SE). m–p) At E17.5, scattered Opn5 expression, but no Opn3 expression, is detected in the respiratory epithelium (RE; indicated with asterisks). Scale bars: a–d) 100 µm; e–h) 200 µm; i–p) 25 µm.
    Opn3 Egfp Reporter Line, supplied by Mutant Mouse Resource & Research Center, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/opn3+egfp+reporter+line/bac+d1+egfp+lines+reporter/pmc12628503-32-10-18
    Average 86 stars, based on 1 article reviews
    opn3 egfp reporter line - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Immunohistochemistry analyzes of Opn3, Opn5, and Tubb3 expression in the nasal epithelium at E13.5 and E17.5 using Opn3 - eGFP and Opn5 -tdTomato mice. a–d) At E13.5, Opn3 is expressed in the Tubb3 + sensory epithelium ( N = 3), while no Opn5 expression is detected in the entire nasal epithelium ( N = 3). e–h) At E17.5, Opn3 is expressed in the Tubb3 + sensory epithelium ( N = 5), and Opn5 is expressed in a few scattered cells in the respiratory epithelium ( N = 5). White boxes indicate magnified images shown in (i–p). In (a, d, e and h), arrows indicate the vomeronasal organ, and arrowheads indicate migratory olfactory neurons. i–l) At e 17.5, Opn3 is co-localized with Tubb3, which marks postmitotic immature neurons, while no expression of Opn5 is detected in the sensory epithelium (SE). m–p) At E17.5, scattered Opn5 expression, but no Opn3 expression, is detected in the respiratory epithelium (RE; indicated with asterisks). Scale bars: a–d) 100 µm; e–h) 200 µm; i–p) 25 µm.

    Journal: Chemical Senses

    Article Title: Opposing expression pattern of opsin 3 and opsin 5 in the developing and adult nasal epithelium

    doi: 10.1093/chemse/bjaf051

    Figure Lengend Snippet: Immunohistochemistry analyzes of Opn3, Opn5, and Tubb3 expression in the nasal epithelium at E13.5 and E17.5 using Opn3 - eGFP and Opn5 -tdTomato mice. a–d) At E13.5, Opn3 is expressed in the Tubb3 + sensory epithelium ( N = 3), while no Opn5 expression is detected in the entire nasal epithelium ( N = 3). e–h) At E17.5, Opn3 is expressed in the Tubb3 + sensory epithelium ( N = 5), and Opn5 is expressed in a few scattered cells in the respiratory epithelium ( N = 5). White boxes indicate magnified images shown in (i–p). In (a, d, e and h), arrows indicate the vomeronasal organ, and arrowheads indicate migratory olfactory neurons. i–l) At e 17.5, Opn3 is co-localized with Tubb3, which marks postmitotic immature neurons, while no expression of Opn5 is detected in the sensory epithelium (SE). m–p) At E17.5, scattered Opn5 expression, but no Opn3 expression, is detected in the respiratory epithelium (RE; indicated with asterisks). Scale bars: a–d) 100 µm; e–h) 200 µm; i–p) 25 µm.

    Article Snippet: Genetically modified mouse strains used were as follows: (i) an Opn3 - eGFP reporter line ( Tg[Opn3-EGFP]JY3Gsat ; MMRRC/GENSAT stock number 030727-UCD, Mutant Mouse Resource & Research Centers, University of California, Davis, California); (ii) an Opn5 Cre/+ ; Ai14 reporter line ( ); (iii) an Opn3 lacZ/lacZ line that functionally acts like an Opn3 −/− line, mixed background strain C57Bl6/6N/FVB/N ( ); and (iv) an Opn5 −/− line, mixed background strain C57/129/CD1/FVB ( ).

    Techniques: Immunohistochemistry, Expressing

    Analyzes of Opn3 -eGFP/ Opn5 -tdTomato double reporter expression in comparison with Tubb3 and Foxj1 double immunohistochemistry at P14 ( N = 4). a–c) At P14, the Opn5 expression has expanded in the respiratory epithelium, with opposing Opn3 expression observed in the sensory epithelium. Most Opn5 + respiratory epithelial cells were observed in the distal part of the nasal cavity. A few intermingled Opn3 + and Opn5 + cells were detected where the sensory and respiratory domains meet (indicated by arrowheads). d) The sensory epithelium defined by Tubb3 expression and the respiratory domain by Foxj1 expression mimic the Opn3 and Opn5 expression domains, respectively. Scale bar: 200 µm.

    Journal: Chemical Senses

    Article Title: Opposing expression pattern of opsin 3 and opsin 5 in the developing and adult nasal epithelium

    doi: 10.1093/chemse/bjaf051

    Figure Lengend Snippet: Analyzes of Opn3 -eGFP/ Opn5 -tdTomato double reporter expression in comparison with Tubb3 and Foxj1 double immunohistochemistry at P14 ( N = 4). a–c) At P14, the Opn5 expression has expanded in the respiratory epithelium, with opposing Opn3 expression observed in the sensory epithelium. Most Opn5 + respiratory epithelial cells were observed in the distal part of the nasal cavity. A few intermingled Opn3 + and Opn5 + cells were detected where the sensory and respiratory domains meet (indicated by arrowheads). d) The sensory epithelium defined by Tubb3 expression and the respiratory domain by Foxj1 expression mimic the Opn3 and Opn5 expression domains, respectively. Scale bar: 200 µm.

    Article Snippet: Genetically modified mouse strains used were as follows: (i) an Opn3 - eGFP reporter line ( Tg[Opn3-EGFP]JY3Gsat ; MMRRC/GENSAT stock number 030727-UCD, Mutant Mouse Resource & Research Centers, University of California, Davis, California); (ii) an Opn5 Cre/+ ; Ai14 reporter line ( ); (iii) an Opn3 lacZ/lacZ line that functionally acts like an Opn3 −/− line, mixed background strain C57Bl6/6N/FVB/N ( ); and (iv) an Opn5 −/− line, mixed background strain C57/129/CD1/FVB ( ).

    Techniques: Expressing, Comparison, Immunohistochemistry

    Immunohistochemistry analyzes of Opn3, Opn5, Foxj1, Ker8, Sox2, Tubb3, and OMP expression in the nasal epithelium at adulthood using Opn3 - eGFP ( N = 5) and Opn5 -tdTomato mice ( N = 4). a, b) In the adult nasal epithelium, an increased number of Opn5 + cells were detected in a scattered pattern within the Foxj1 + respiratory epithelium. White box in (a) indicates the magnified image shown in b. c–e) In the olfactory sensory epithelium, Opn3 and Ker8 are co-expressed in sustentacular cells. f) Opn3 is co-expressed with Sox2 in sustentacular cell nuclei (indicated by an arrow), but seemingly not in Sox2 + globose basal cells (indicated by arrowheads). g, h) Opn3 is not expressed in Tubb3 + postmitotic immature ORNs (g) or OMP + mature ORNs (h). Scale bars: a) 200 µm; b) 100 µm; c–h) 50 µm.

    Journal: Chemical Senses

    Article Title: Opposing expression pattern of opsin 3 and opsin 5 in the developing and adult nasal epithelium

    doi: 10.1093/chemse/bjaf051

    Figure Lengend Snippet: Immunohistochemistry analyzes of Opn3, Opn5, Foxj1, Ker8, Sox2, Tubb3, and OMP expression in the nasal epithelium at adulthood using Opn3 - eGFP ( N = 5) and Opn5 -tdTomato mice ( N = 4). a, b) In the adult nasal epithelium, an increased number of Opn5 + cells were detected in a scattered pattern within the Foxj1 + respiratory epithelium. White box in (a) indicates the magnified image shown in b. c–e) In the olfactory sensory epithelium, Opn3 and Ker8 are co-expressed in sustentacular cells. f) Opn3 is co-expressed with Sox2 in sustentacular cell nuclei (indicated by an arrow), but seemingly not in Sox2 + globose basal cells (indicated by arrowheads). g, h) Opn3 is not expressed in Tubb3 + postmitotic immature ORNs (g) or OMP + mature ORNs (h). Scale bars: a) 200 µm; b) 100 µm; c–h) 50 µm.

    Article Snippet: Genetically modified mouse strains used were as follows: (i) an Opn3 - eGFP reporter line ( Tg[Opn3-EGFP]JY3Gsat ; MMRRC/GENSAT stock number 030727-UCD, Mutant Mouse Resource & Research Centers, University of California, Davis, California); (ii) an Opn5 Cre/+ ; Ai14 reporter line ( ); (iii) an Opn3 lacZ/lacZ line that functionally acts like an Opn3 −/− line, mixed background strain C57Bl6/6N/FVB/N ( ); and (iv) an Opn5 −/− line, mixed background strain C57/129/CD1/FVB ( ).

    Techniques: Immunohistochemistry, Expressing

    Buried food tests using Opn3 or Opn5 wild-type and deficient mice. Olfaction was evaluated by the buried food test, with a maximum of 10 min (i.e. 600 s) per trial to find a hidden cookie piece. a–c) The graphs show the time to find buried food for Opn3 +/+ ( N = 10) and Opn3 −/− mice ( N = 9). a) The bars represent the average time of three independent trials. b) The bars represent the three individual trials, T1–T3, analyzed as a function of trial order. c) The bars represent time to find buried food between T1 and T3 for Opn3 +/+ mice ( P = 0.0076), and Opn3 −/− mice ( P = 0.097). d–f) The graphs show the time to find buried food for Opn5 +/+ ( N = 8) and Opn5 −/− mice ( N = 11). (d) The bars represent the average time of three independent trials. e) The bars represent the three individual trials, T1–T3. f) The bars represent time to find buried food between T1 and T3 for Opn5 +/+ mice ( P = 0.72) and Opn5 −/− mice ( P = 0.21). Data on all graphs are presented as the mean ± standard deviation. Statistical significances were calculated using unpaired two-tailed Student's t -tests (a, c, d, f) or repeated measures two-way ANOVA followed by Sidak's multiple comparisons test (b, e).

    Journal: Chemical Senses

    Article Title: Opposing expression pattern of opsin 3 and opsin 5 in the developing and adult nasal epithelium

    doi: 10.1093/chemse/bjaf051

    Figure Lengend Snippet: Buried food tests using Opn3 or Opn5 wild-type and deficient mice. Olfaction was evaluated by the buried food test, with a maximum of 10 min (i.e. 600 s) per trial to find a hidden cookie piece. a–c) The graphs show the time to find buried food for Opn3 +/+ ( N = 10) and Opn3 −/− mice ( N = 9). a) The bars represent the average time of three independent trials. b) The bars represent the three individual trials, T1–T3, analyzed as a function of trial order. c) The bars represent time to find buried food between T1 and T3 for Opn3 +/+ mice ( P = 0.0076), and Opn3 −/− mice ( P = 0.097). d–f) The graphs show the time to find buried food for Opn5 +/+ ( N = 8) and Opn5 −/− mice ( N = 11). (d) The bars represent the average time of three independent trials. e) The bars represent the three individual trials, T1–T3. f) The bars represent time to find buried food between T1 and T3 for Opn5 +/+ mice ( P = 0.72) and Opn5 −/− mice ( P = 0.21). Data on all graphs are presented as the mean ± standard deviation. Statistical significances were calculated using unpaired two-tailed Student's t -tests (a, c, d, f) or repeated measures two-way ANOVA followed by Sidak's multiple comparisons test (b, e).

    Article Snippet: Genetically modified mouse strains used were as follows: (i) an Opn3 - eGFP reporter line ( Tg[Opn3-EGFP]JY3Gsat ; MMRRC/GENSAT stock number 030727-UCD, Mutant Mouse Resource & Research Centers, University of California, Davis, California); (ii) an Opn5 Cre/+ ; Ai14 reporter line ( ); (iii) an Opn3 lacZ/lacZ line that functionally acts like an Opn3 −/− line, mixed background strain C57Bl6/6N/FVB/N ( ); and (iv) an Opn5 −/− line, mixed background strain C57/129/CD1/FVB ( ).

    Techniques: Standard Deviation, Two Tailed Test

    Immunohistochemistry analyzes of Tubb3, Foxj1, OMP, Sox2 and Ker8 in the nasal cavity of adult Opn3 +/+ , Opn3 −/− , Opn5 +/+ , and Opn5 −/− mice ( N = 3 for each mouse genotype). a–h) No apparent differences in the expression of Tubb3, Foxj1, OMP, Sox2, or Ker8 in the sensory epithelium of Opn3 −/− compared to Opn3 +/+ mice. e, f) Arrows indicate the Sox2 + sustentacular cells, and arrowheads indicate Sox2 + globose basal cells. i–l) No apparent difference in the expression of Tubb3, Foxj1, or Sox2 in the respiratory epithelium of Opn5 −/− compared to Opn5 +/+ mice. k, l) Arrows indicate the Sox2 + positive cells in the respiratory cell layer. Scale bars: a, b, i, j) 100 µm; c–h, k, l) 50 µm.

    Journal: Chemical Senses

    Article Title: Opposing expression pattern of opsin 3 and opsin 5 in the developing and adult nasal epithelium

    doi: 10.1093/chemse/bjaf051

    Figure Lengend Snippet: Immunohistochemistry analyzes of Tubb3, Foxj1, OMP, Sox2 and Ker8 in the nasal cavity of adult Opn3 +/+ , Opn3 −/− , Opn5 +/+ , and Opn5 −/− mice ( N = 3 for each mouse genotype). a–h) No apparent differences in the expression of Tubb3, Foxj1, OMP, Sox2, or Ker8 in the sensory epithelium of Opn3 −/− compared to Opn3 +/+ mice. e, f) Arrows indicate the Sox2 + sustentacular cells, and arrowheads indicate Sox2 + globose basal cells. i–l) No apparent difference in the expression of Tubb3, Foxj1, or Sox2 in the respiratory epithelium of Opn5 −/− compared to Opn5 +/+ mice. k, l) Arrows indicate the Sox2 + positive cells in the respiratory cell layer. Scale bars: a, b, i, j) 100 µm; c–h, k, l) 50 µm.

    Article Snippet: Genetically modified mouse strains used were as follows: (i) an Opn3 - eGFP reporter line ( Tg[Opn3-EGFP]JY3Gsat ; MMRRC/GENSAT stock number 030727-UCD, Mutant Mouse Resource & Research Centers, University of California, Davis, California); (ii) an Opn5 Cre/+ ; Ai14 reporter line ( ); (iii) an Opn3 lacZ/lacZ line that functionally acts like an Opn3 −/− line, mixed background strain C57Bl6/6N/FVB/N ( ); and (iv) an Opn5 −/− line, mixed background strain C57/129/CD1/FVB ( ).

    Techniques: Immunohistochemistry, Expressing